Brooks, S.P.J. and Storey, K.B. 1988. The glycolytic enzyme complex: preconception or experimental conclusion. Biochemistry (Life Sci. Adv.) 7, 439- 453.

The glycolytic enzyme complex: Preconception or experimental conclusion.

S.P.J. Brooks and K.B. Storey

Several different experimental protocols have been used to demonstrate the existence of a functional glycolytic complex, defined as an organized enzyme structure capable of metabolizing glucose at rates faster than the soluble enzymes. The majority of these studies are structural; measuring physical interactions between individual pairs of enzymes; and between enzymes and actin. Unfortunately, these associations are measured at low pH and low ionic strength values conditions which are far from physiological, and which ensure the existence of these complexes. Furthermore, these studies fail to demonstrate that enzymes are organized into a functional complex since they measured only increased enzyme binding. Kinetic studies of enzyme-enzyme and enzyme-actin binding shows that enzymes are inhibited when bound (except phosphofructokinase) demonstrating that, when complexes have been identified, they exhibit properties contrary to theoretical predictions. The data indicate that a functional glycolytic complex does not exist in the muscle tissues studied to date.