The Aitken Lab
Lodha PH, Hopwood EM, Manders AL, Aitken SM
Residue N84 of yeast cystathionine beta-synthase is a determinant of reaction specificity.
Biochim. Biophys. Acta 2010. in press.
Lodha PH, Jaworski AF, Aitken SM
Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of Escherichia coli cystathionine beta-lyase.
Protein Sci. 2010. 19(3):383-91.
Lodha PH, Shadnia H, Woodhouse CM, Wright JS, Aitken SM
Investigation of residues Lys112, Glu136, His138, Gly247, Tyr248, and Asp249 in the active site of yeast cystathionine beta-synthase.
Biochem. Cell Biol. 2009. 87(3):531-40.
Farsi A, Lodha PH, Skanes JE, Los H, Kalidindi N, Aitken SM
Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms and reaction specificity.
Biochem. Cell Biol. 2009. 87(2):445-57.
Quazi FI, Aitken SM
Characterization of the S289A,D mutants of yeast cystathionine beta-synthase.
Biochim. Biophys. Acta. 2009. 1794(6):892-7.
Belew MS, Quazi FI, Willmore WG, Aitken SM
Kinetic characterization of recombinant human cystathionine beta-synthase purified from E. coli.
Protein Expr. Purif. 2009. 64(2):139-45.
Aitken SM, Kirsch JF
The enzymology of cystathionine biosynthesis: strategies for the control of substrate and reaction specificity.
Arch. Biochem. Biophys. 2005. 433(1):166-75.
Aitken SM, Kirsch JF
Role of active-site residues Thr81, Ser82, Thr85, Gln157, and Tyr158 in yeast cystathionine beta-synthase catalysis and reaction specificity.
Biochemistry. 2004. 43(7):1963-71.
Ouellet M, Aitken SM, English AM, Percival MD
Aromatic hydroxamic acids and hydrazides as inhibitors of the peroxidase acitivity of prostaglandin H2 synthase-2.
Arch. Biochem. Biophys. 2004. 431(1):107-18.
Aitken SM, Kim DH, Kirsch JF
Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions.
Biochemistry. 2003. 42(38):11297-306.
Aitken SM, Kirsch JF
Kinetics of the yeast cystathionine beta-synthase forward and reverse reactions: continuous assays and the equilibrium constant for the reaction.
Biochemistry. 2003. 42(2):571-8.
Aitken SM, Ouellet M, Percival MD, English AM
Mechanism of horseradish peroxidase inactivation by benzhydrazide: a critical evaluation of arylhydrazides as peroxidase inhibitors.
Biochem. J. 2003. 375(Pt 3):613-21.
Aitken SM, Turnbull JL, Percival MD, English AM
Thermodynamic analysis of the binding of aromatic hydroxamic acid analogues to ferric horseradish peroxidase.
Biochemistry. 2001.40(46):13980-9.
Aitken SM, Attucci S, Ibrahim RK, Gulick PJ
A cDNA encoding geranylgeranyl pyrophosphate synthase from white lupin.
Plant Physiol. 1995. 108(2):837-8.
Attucci S, Aitken SM, Ibrahim RK, Gulick PJ
A cDNA encoding farnesyl pyrophosphate synthase in white lupin.
Plant Physiol. 1995. 108(2):835-6.
Attucci S, Aitken SM, Gulick PJ, Ibrahim RK
Farnesyl pyrophosphate synthase from white lupin: molecular cloning, expression, and purification of the expressed protein.
Arch. Biochem. Biophys. 1995. 321(2):493-500.
Aitken SM, Tinker N, Mather DE, Fortin MG
A method for detecting DNA polymorphism in large populations.
Genome. 1994. 37(3):506-8.
Last updated April 16, 2010